Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Sp6

Cell type

Cell type Class
Others
Cell type
Dental mesenchyme
NA
NA

Attributes by original data submitter

Sample

source_name
dental mesenchyme
strain
C57BL/6
tissue
dental mesenchyme
chip antibody
Sp6 antibody (in house)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Dental epithelium and dental esenchyme from 30 newborn C57BL/6 P1 molars werer isolated. After crosslinking with PFA, tissue ysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with epiprofin/Sp6 antibody. Libraries were prepared by Genpathway according to Illumina's instructions. Libraries were sequenced on the Genome Analyzer 2 following the manufacturer's protocols.

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

mm10

Number of total reads
53946647
Reads aligned (%)
83.8
Duplicates removed (%)
33.0
Number of peaks
7102 (qval < 1E-05)

mm9

Number of total reads
53946647
Reads aligned (%)
83.6
Duplicates removed (%)
33.1
Number of peaks
7088 (qval < 1E-05)

Base call quality data from DBCLS SRA